Ham's F12 Medium

 

In the past Ham`s F12 was the first choice for a serum-free cultivation of CHO-cells and is now substituted through better serum-free systems like our Panserin C6000, which is protein-free in addition. However, Ham`s F12 is an appropriate medium for mammalian cells when it is supplemented with FBS. It contains a high concentration of vitamins, amino acids and trace elements. The content of zinc sulfate is increased and it contains putrescine and linoleic acid.




DescriptionSizeProduct number
Ham`s F12 Medium
without L-Glutamine
with 1.176 g/L NaHCO3
500 ml ST04-14550
Ham`s F12 Medium
with L-Glutamine
with 1.176 g/L NaHCO3
500 ml ST04-14500
Ham`s F12 Medium
with stable Glutamine
with 1.176 g/L NaHCO3
500 ml ST04-15500
 

 


DescriptionSizeProduct number
Ham`s F12 Medium
with L-Glutamine
without Phenol red
with 25 mM HEPES
with 1.176 g/L NaHCO3
500 ml ST04-14501
Ham`s F12 Medium
without L-Glutamine
without Phenol red
with 1.176 g/L NaHCO3
500 ml ST04-14559
Ham`s F12K Medium
with L-Glutamine
with 2.5 g/L NaHCO3
500 ml ST04-15600
 
Minimum order 20 pieces

DescriptionSizeProduct number
Ham`s F12 Medium
without L-Glutamine
without NaHCO3
10 L
50 L
ST03-4110
ST03-4150
 

 


 Componentsmg/L
Inorganic Salts Calcium chloride anhydrous
Copper(II) sulfate x 5H2O
Iron(II) sulfate x 7H2O
Magnesium chloride x 6H2O
Potassium chloride
Sodium chloride
di-Sodium hydrogen phosphate anhydrous
Zinc sulfate x 7H2O
33.30
0.003
0.834
122.00
223.65
7599.9
142.04
0.86
Other Components D(+)-Glucose anhydrous
HEPES
Hypoxanthine
Linoleic acid
DL-Lipoic acid
Phenol red
Putrescine x 2HCl
Sodium pyruvate
Thymidine
1,801.60
5,958.00
4.08
0.084
0.21
1.20
0.16
110.00
0.73
Amino Acids L-Alanine
L-Arginine x HCl
L-Asparagine x H2O
L-Aspartic acid
L-Cysteine x HCl x H2O
L-Glutamine
L-Glutamic acid
Glycine
L-Histidine x HCl x H2O
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
8.91
210.70
15.01
13.31
35.12
146.20
14.71
7.51
20.96
3.94
13.12
36.54
4.48
4.96
34.53
10.51
11.91
2.04
5.44
11.71
Vitamins D(+)-Biotin
D-Calcium pantothenate
Choline chloride
Folic acid
myo-Inositol
Nicotinamide
Pyridoxine x HCl
Riboflavin
Thiamine x HCl
Vitamin B12
0.007
0.24
13.96
1.32
18.00
0.037
0.062
0.03
0.34
1.36

When 5,958.00 mg/L HEPES is included there is only 7,099.00 mg/L sodium chloride.


 L-GlutamineStable GlutaminePhenol redHEPESSpecial
ST04-14550     x    
ST04-14500 x   x    
ST04-15500   x x    
ST04-14501 x     25 mM See above
ST04-14559         See above
ST04-15600 x   x   See above
This website use cookies to create the most secure and effective website. By continuing to use this website you agree that cookies are used.
More information Ok