Iscove's Modified Dulbecco's Medium

 

The IMDM is a modified DMEM, with a higher content of vitamins, selenium and amino acids. As it is supplemented with albumin, transferrin and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells or hybridoma cells. Note: for hybridomas there is a better and highly efficient protein-free medium available: our Panserin H4000.

 


DescriptionSizeProduct number
IMDM
without L-Glutamine
with 3.024 g/L NaHCO3
500 ml ST04-20250
IMDM
with L-Glutamine
with 3.024 g/L NaHCO3
500 ml ST04-20350
IMDM
without L-Glutamine
with 25 mM HEPES
with 3.024 g/L NaHCO3
500 ml ST04-20050
IMDM
with L-Glutamine
with 25 mM HEPES
with 3.024 g/L NaHCO3
500 ml ST04-20150
 

 


DescriptionSizeProduct number
IMDM
with L-Glutamine
with 1.0 g/L Glucose
with 3.024 g/L NaHCO3
500 ml ST04-20259
IMDM
with stable Glutamine
with 25 mM HEPES
without Phenol red
315 mOsm*
with 3.024 g/L NaHCO3
500 ml ST04-20451S1
IMDM
with L-Glutamine
with 25 mM HEPES
320 mOsm*
with 3.024 g/L NaHCO3
500 ml ST04-20150S2
IMDM
with L-Glutamine
with 1.5 g/L NaHCO3
500 ml ST04-20351
IMDM
with stable Glutamine
with 25 mM HEPES
with 3.024 g/L NaHCO3
500 ml ST04-20450
IMDM
with stable Glutamine
with 25 mM HEPES
without Phenol red
with 3.024 g/L NaHCO3
500 ml ST04-20451
 
* Due to osmolality, the salt concentration can vary.

  Minimum order 20 pieces

DescriptionSizeProduct number
IMDM
without L-Glutamine
without NaHCO3
10 L
50 L
ST03-5210
ST03-5250
IMDM
with L-Glutamine
without NaHCO3
10 L
50 L
ST03-1310
ST03-1350
IMDM
with L-Glutamine
with 25 mM HEPES
without NaHCO3
10 L
50 L
ST03-1410
ST03-1450
 

 


 Components
mg/L
Inorganic Salts





Calcium chloride anhydrous
Potassium chloride
Potassium nitrate
Magnesium sulfate anhydrous
Sodium chloride
Sodium dihydrogen phosphate x H2O
Sodium selenite x 5H2O
165.00
330.00
0.076
97.66
5,005.00
125.00
0.01
Other Components D(+)-Glucose anhydrous
HEPES
Sodium pyruvate
Phenol red
4,500.00
5,958.00
110.00
15.00
AminoAcids L-Alanine
L-Arginine x HCl
L-Asparagine x H2O
L-Aspartic acid
L-Cystine x 2HCl
L-Glutamine
L-Glutamic acid
Glycine
L-Histidine x HCl x H2O
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine x 2Na x 2H2O
L-Valine
25.00
84.00
28.40
30.00
91.24
584.00
75.00
30.00
42.00
105.00
105.00
146.00
30.00
66.00
40.00
42.00
95.00
16.00
104.2
94.00
Vitamins D(+)-Biotin
D-Calcium pantothenate
Choline chloride
Folic acid
myo-Inositol
Nicotinamide
Pyridoxine x HCl
Riboflavin
Thiamine x HCl
Vitamin B12
0.0130
4.00
4.00
4.00
7.20
4.00
4.00
0.40
4.00
0.013

When 5,958.00 mg/L HEPES is included there is only 4,505.00 mg/L
sodium chloride.


 L-GlutamineStable GlutaminePhenol redHEPESSpecial
ST04-20250     x    
ST04-20350 x   x    
ST04-20050     x 25 mM  
ST04-20150 x   x 25 mM  
ST04-20259     x   See above
ST04-20451S1   x   25 mM See above
ST04-20150S2 x   x 25 mM See above
ST04-20351 x   x   See above
ST04-20450   x x 25 mM See above
ST04-20451   x   25 mM See above
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