MCDB Medium

 

MCDB 131 is a medium for the cultivation of human micro-vascular endothelial cells under reduced serum content. For this purpose it has be supplemented with dialyzed serum, EGF and hydrocortisone.




DescriptionSizeProduct number
MCDB 131
without L-Glutamine
with 1.176 g/L NaHCO3
500 ml ST04-80057
 

DescriptionSizeProduct number
MCDB 131
with L-Glutamine
with 1.176 g/L NaHCO3
500 ml ST04-80053
MCDB 131
without Glutamine
with 25 mM HEPES
with 1.176 g/L NaHCO3
500 ml ST04-80054

Minimum order 20 pieces

 Componentsmg/L
Inorganic Salts Ammonium metavanadate
Calcium chloride x 2H2O
Copper(II) sulfate x 5H2O
Iron (III) sulfate x 7H2O
Magnesium sulfate anhydrous
Manganese chloride x 4H2O
Ammonium molybdate x 4H2O
Nickel chloride x 6H2O
Potassium chloride
Sodium chloride
Sodium metasilicate
di-Sodium hydrogen phosphate
Sodium selenite anhydrous
Zinc sulfate x 7H2O
0.0006
235.05
0.0012
0.283
1,204.00
0.0002
0.0037
0.0007
298.00
6,430.00
2.09
71.00
0.0052
0.0003
Other Components






Adenine
D-Glucose
DL-α-Lipoic acid
HEPES
Phenol red
Putrescine x 2HCl
Sodium pyruvate
2`-Deoxythymidine
0.135
1,000.00
0.0021
5,958.00
10.00
0.002
110.00
0.024
Amino Acids



















L-Alanine
L-Arginine x HCl
L-Asparagine x H2O
L-Aspartic acid
L-Cysteine x HCl x H2O
L-Glutamic acid
L-Glutamine
Glycine
L-Histidine x HCl x H2O
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
2.70
63.20
15.00
13.30
35.00
4.00
1,461.00
2.30
42.00
66.00
131.00
182.00
15.00
33.00
11.50
32.00
12.00
4.10
18.10
117.10
Vitamins









Choline Chloride
D(+)-Biotin
Folic Acid
myo-Inositol
Niacinamide
D-Calcium pantothenate
Pyridoxine x HCl
Riboflavin
Thiamine x HCl
Vitamin B12
13.96
0.0073
0.60
7.20
6.10
12.00
2.10
0.0038
3.40
0.0136
If 5,958.00 mg/L HEPES are included there are only 4,400.00 mg/L sodium chloride.
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