Medium 199 with Earle's Salts

 

The M199 was originally developed to assay the nutrient demand of embryonic chicken fibroblasts. But it works very well with cells from many different animal species. For example, it is used for vaccine production in virology. For long term cultures serum should be added.




DescriptionSizeProduct number
M199 with EBSS
without L-Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-07500
 

DescriptionSizeProduct number
M199 with EBSS
with L-Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-07050
M199 with EBSS
with stable Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-07250
M199 with EBSS
with L-Glutamine
with 25 mM HEPES
with 2.2 g/L NaHCO3
500 ml ST04-07150

Minimum order 20 pieces

DescriptionSizeProduct number
M199 with EBSS
with L-Glutamine
without NaHCO3
10 L
50 L
ST03-1910
ST03-1950
 

 Componentsmg/L
Inorganic Salts






Calcium chloride x 2H2O
Iron(III) nitrate x 9H2O
Magnesium sulfate anhydrous
Potassium chloride
Sodium acetate x 3H2O
Sodium chloride
Sodium dihydrogen phosphate
264.92
0.72
97.67
400.00
82.95
6,800.00
140.00
Other Components














Adenine sulfate
AMP
ATP
Cholesterol
2`-Deoxyribose
D(+)-Glucose anhydrous
Glutathione (red.)
Guanine x HCl
HEPES
Hypoxanthine
Phenol red
D-Ribose
Thymine
Tween 80
Uracil
Xanthine
10.00
0.20
1.00
0.20
0.50
1,000.00
0.05
0.30
5,958.00
0.30
10.00
0.50
0.30
4.90
0.30
0.30
Amino Acids



















L-Alanine
L-Arginine x HCl
L-Aspartic acid
L-Cysteine x HCl x H2O
L-Cystine
L-Glutamine
L-Glutamic acid
Glycine
L-Histidine x HCl x H2O
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
25.00
70.00
30.00
0.10
20.00
100.00
67.00
50.00
21.88
10.00
20.00
60.00
70.00
15.00
25.00
40.00
25.00
30.00
10.00
40.00
25.00
Vitamins

















p-Aminobenzoic acid
Ascorbic acid
D(+)-Biotin
Calciferol
D-Calcium pantothenate
Choline chloride
Folic acid
myo-Inositol
Menadione
Nicotinic acid
Nicotinamide
Pyridoxal x HCl
Pyridoxol x HCl
Riboflavin
DL-α-Tocopherol phosphate disodium
salt
Thiamine x HCl
Vitamin A acetate
0.05
0.05
0.01
0.10
0.01
0.50
0.01
0.05
0.01
0.025
0.025
0.025
0.025
0.01
0.01

0.01
0.14

When 5,958.00 mg/L HEPES is included there is only 6,300.00 mg/L sodium chloride.
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