Medium 199 with Hank's Salts

 

The M199 was originally developed to assay the nutrient demand of embryonic chicken fibroblasts. But it works very well with cells from many different animal species. For example, it is used for vaccine production in virology. For long term cultures serum should be added.




DescriptionSizeProduct number
M199 with HBSS
without L-Glutamine
with 0.35 g/L NaHCO3
500 ml ST04-07753
M199 with HBSS
with L-Glutamine
with 25 mM HEPES
with 0.35 g/L NaHCO3
500 ml ST04-07450
M199 with HBSS (10X)
without L-Glutamine
without NaHCO3
500 ml ST04-07600

Minimum order 20 pieces

DescriptionSizeProduct number
M199 with HBSS
with L-Glutamine
without NaHCO3
10 L
50 L
ST03-2110
ST03-2150
 

 Componentsmg/L
Inorganic Salts







Calcium chloride x 2H2O
Iron(III) nitrate x 9H2O
Magnesium sulfate anhydrous
Potassium chloride
Potassium dihydrogen phosphate
Sodium acetate
Sodium chloride
di-Sodium hydrogen phosphate
185.45
0.72
97.67
400.00
60.00
50.03
8,000.00
47.68
Other Components














Adenine sulfate
AMP
ATP
Cholesterol
2`-Deoxyribose
D(+)-Glucose anhydrous
Glutathione (red.)
Guanine x HCl
HEPES
Hypoxanthine
Phenol red
D-Ribose
Thymine
Tween 80
Uracil
Xanthine
10.00
0.20
1.00
0.20
0.50
1,000.00
0.05
0.30
5,958.00
0.30
10.00
0.50
0.30
4.90
0.30
0.30
Amino Acids




















L-Alanine
L-Arginine x HCl
L-Aspartic acid
L-Cysteine x HCl x H2O
L-Cystine
L-Glutamine
L-Glutamic acid
Glycine
L-Histidine x HCl x H2O
L-Hydroxyproline
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
25.00
70.00
30.00
0.10
20.00
100.00
67.00
50.00
21.88
10.00
20.00
60.00
70.00
15.00
25.00
40.00
25.00
30.00
10.00
40.00
25.00
Vitamins p-Aminobenzoic acid
Ascorbic acid
D(+)-Biotin
Calciferol
D-Calcium pantothenate
Choline chloride
Folic acid
myo-Inositol
Menadione
Nicotinic acid
Nicotinamide
Pyridoxal x HCl
Pyridoxol x HCl
Riboflavin
DL-α-Tocopherol phosphate-disodium salt
Thiamine x HCl
Vitamin A acetate
0.05
0.05
0.01
0.10
0.01
0.50
0.01
0.05
0.01
0.025
0.025
0.025
0.025
0.01
0.01
0.01
0.14
When 5,958.00 mg/L HEPES is included there is only 7,500.00 mg/L sodium chloride.
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