MEM with Earle's Salts

 

MEM is an advancement of the BME and the base medium of many further modifications. Because BME did not fulfil all requirements for some mammalian and HeLa cells, a better variation had to be developed. Today, MEM is one of the most used synthetic media and shows its versatility by supplementing with amino acids including Hank`s or Earle`s salts. Even the addition of only small amounts of FBS results in a positive effect on cell growth.




DescriptionSizeProduct number
MEM Eagle with EBSS
without L-Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-08050
MEM Eagle with EBSS
without L-Glutamine
with 25 mM HEPES
with 2.2 g/L NaHCO3
500 ml ST04-08150
MEM Eagle with EBSS
without L-Glutamine
with NEAA*
with 2.2 g/L NaHCO3
500 ml ST04-08509

 Componentsmg/L
Amino Acids






L-Alanine
L-Asparagine x H2O
L-Aspartic acid
L-Glutamic acid
L-Glycine
L-Proline
L-Serine
8.90
13.20
13.30
14.70
7.50
11.50
10.50


 

DescriptionSizeProduct number
MEM Eagle with EBSS
without L-Glutamine
without Phenol red
with 2.2 g/L NaHCO3
500 ml ST04-00507
MEM Eagle with EBSS
without L-Glutamine
without NaHCO3
500 ml ST04-09050

Minimum order 20 pieces

DescriptionSizeProduct number
MEM Eagle with EBSS
without L-Glutamine
without NaHCO3
10 L
50 L
ST03-7410
ST03-7450
 

DescriptionSizeProduct number
MEM Eagle with EBSS
with L-Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-08500
MEM Eagle with EBSS
with L-Glutamine
with 1.5 g/L NaHCO3
500 ml ST04-00509
MEM Eagle with EBSS
with 2 mM L-Glutamine
with 1 mM Pyruvate
with NEAA*
with 1.5 g/L NaHCO3
500 ml ST04-08056

 Componentsmg/L
Amino Acids






L-Alanine
L-Asparagine x H2O
L-Aspartic acid
L-Glutamic acid
L-Glycine
L-Proline
L-Serine
8.90
13.20
13.30
14.70
7.50
11.50
10.50
  
 

DescriptionSizeProduct number
MEM Eagle with EBSS
with L-Glutamine
without Phenol red
with 1.5 g/L NaHCO3
500 ml ST04-00508
MEM Eagle with EBSS
with L-Glutamine
with 20 mM HEPES
with 2.2 g/L NaHCO3
500 ml ST04-08549
MEM Eagle with EBSS
with L-Glutamine
with NEAA*
with 2.2 g/L NaHCO3
500 ml ST04-08510

 Componentsmg/L
Amino Acids






L-Alanine
L-Asparagine x H2O
L-Aspartic acid
L-Glutamic acid
L-Glycine
L-Proline
L-Serine
8.90
13.20
13.30
14.70
7.50
11.50
10.50
  
 
 
Minimum order 20 pieces

DescriptionSizeProduct number
MEM Eagle with EBSS
with L-Glutamine
with NEAA 
without NaHCO3
10 L
50 L
ST03-2910
ST03-2950
MEM Eagle with EBSS
with L-Glutamine
with NEAA
with 25 mM HEPES 
without NaHCO3
10 L
50 L
ST03-3010
ST03-3050
MEM Eagle with EBSS
with L-Glutamine
without NaHCO3
10 L
50 L
ST03-2710
ST03-2750
MEM Eagle with EBSS
with L-Glutamine
with 25 mM HEPES 
without NaHCO3
10 L
50 L
ST03-2810
ST03-2850
 

DescriptionSizeProduct number
MEM Eagle with EBSS
with stable Glutamine
with 2.2 g/L NaHCO3
500 ml ST04-09500
MEM Eagle with EBSS
with stable Glutamine
with 25 mM HEPES
with 2.2 g/L NaHCO3
500 ml ST04-08250
 

 Componentsmg/L
Inorganic Salts Calcium chloride x 2H2O
Magnesium sulfate anhydrous
Potassium chloride
Sodium chloride
Sodium dihydrogen phosphate x H2O
264,92
97,67
400,00
6,800,00
140,00
Other Components D(+)-Glucose anhydrous
HEPES
Phenol red
1,000,00
5,958,00
10,00
Amino Acids L-Arginine x HCl
L-Cystine
L-Histidine x HCl x H2O
L-Isoleucine
L-Leucine
L-Lysine x HCl
L-Methionine
L-Phenylalanine
L-Threonine
L-Tryptophan
L-Tyrosine
L-Valine
126,00
24,00
42,00
52,00
52,00
72,50
15,00
32,00
48,00
10,00
36,00
46,00
Vitamins D-Calcium pantothenate
Choline chloride
Folic acid
myo-Inositol
Nicotinamide
Pyridoxal x HCl
Riboflavin
Thiamine x HCl
1,00
1,00
1,00
2,00
1,00
1,00
0,10
1,00
If 5,958.00 mg/L HEPES are included there are only 6.300,00 mg/L sodium chloride.


Table for MEM EBSS without Glutamine

 L-GlutamineStable GlutamineNEAAPhenol redHEPESSpecial
ST04-08050       x    
ST04-08150       x 25 mM  
ST04-08509     x x    
ST04-00507           See above
ST04-09050       x   See above

Table for MEM EBSS with L-Glutamine

 L-GlutamineStable GlutamineNEAAPhenol redHEPESSpecial
ST04-08500 x     x    
ST04-00509 x     x    
ST04-08056 x   x x    
ST04-00508 x         See above
ST04-08549 x     x 20 mM See above
ST04-08510 x   x x   See above

Table for MEM EBSS with stable Glutamine

 L-GlutamineStable GlutamineNEAAPhenol redHEPESSpecial
ST04-09500   x   x    
ST04-08250   x   x 25 mM  
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