Proteinase K

 

Proteinase K is an enzyme used to digest most proteins in molecular-biological techniques. The enzyme may be used at 56° C for up to 4 hours, or 37° C for overnight incubations. Proteinase K solution is stabilized with a specially formulated buffer, and can be used directly from the freezer.

 

DescriptionSizeProduct number
Proteinase K 100 mg ST-4300002
 
  • Broad spectrum of serine protease
  • Active under denaturing conditions
  • Stable at high temperatures
  • Molecular biology grade
  • Available as powder and stabilized stock solution
  • Inactivation of RNases/DNases during nucleic acid extraction
  • Protein modification
  • General protein digestion
  • Determination of enzyme localization
  • Dissolve to 20 mg/ml in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
  • Proteinase K may be used at 56° C for up to 4 hours, or 37° C for overnight incubations
  • Proteinase K has an optimal pH of 7.5 - 12.0
  • To remove common contaminants from nucleic acid preparations use at a working concentration of 5 μg/ ml
Proteinase K can be stored for 12 months at -20° C.
RNase Activity: No detectable ribonuclease activity detected with MS2RNA after 6 hour incubation at 37° C DNase Activity: No detectable nicking activity detected with pBR322 after 6 hour incubation at 37° C
One unit is defined as the amount of enzyme that will liberate 1.0 μmol of tyrosine per minute at 37° C, pH 7.5.
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