Proteinase K is an enzyme used to digest most proteins in molecular-biological techniques. The enzyme may be used at 56° C for up to 4 hours, or 37° C for overnight incubations. Proteinase K solution is stabilized with a specially formulated buffer, and can be used directly from the freezer.
|Proteinase K||100 mg||ST-4300002|
- Broad spectrum of serine protease
- Active under denaturing conditions
- Stable at high temperatures
- Molecular biology grade
- Available as powder and stabilized stock solution
- Inactivation of RNases/DNases during nucleic acid extraction
- Protein modification
- General protein digestion
- Determination of enzyme localization
- Dissolve to 20 mg/ml in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
- Proteinase K may be used at 56° C for up to 4 hours, or 37° C for overnight incubations
- Proteinase K has an optimal pH of 7.5 - 12.0
- To remove common contaminants from nucleic acid preparations use at a working concentration of 5 μg/ ml
Proteinase K can be stored for 12 months at -20° C.
RNase Activity: No detectable ribonuclease activity detected with MS2RNA after 6 hour incubation at 37° C DNase Activity: No detectable nicking activity detected with pBR322 after 6 hour incubation at 37° C
One unit is defined as the amount of enzyme that will liberate 1.0 μmol of tyrosine per minute at 37° C, pH 7.5.