PAN Hot Start DNA Polymerase


PAN Hot Start is a heat-activated thermostable DNA polymerase isolated from a novel organism. PAN Hot Start provides improved specificity as compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. PAN Hot Start is inactive at room temperature and therefore, prior to PCR cycling, requires activation by heat treatment for 10 minutes. Subsequently, the reaction can be handled according to the user`s existing protocols for thermostable DNA polymerases. Specificity and performance of PAN Hot Start can be further improved with the use of 2x PAN Mate Additive, which is designed for GC- or AT-rich DNA, „dirty“ templates or sequences with a high level of secondary structure.


DescriptionSizeProduct number
PAN Hot Start DNA Polymerase 250 units
500 units
5000 units
  • Outstanding and robust performance
  • For PCR assays requiring hot-start
  • Excellent yield in quantitative assays
  • Convenient set up at room temperature
  • Leaves “A“ overhang
  • Available as ready-to-go versions PAN Hot Mix and PAN Hot Mix red
  • Highly suited to real-time assays
  • Products suitable for TA cloning
10x PAN Hot Start Buffer                     5 μl
50 mM MgCl2                                     1.5 - 4.0 μl
100 mM dNTP Mix (see below)             0.5 - 1.0 μl
Template and Primers                          as required
PAN Hot Start                                     0.2 - 1.0 μl
Water (ddH2O)                                    up to 50 μl 

100 mM dNTP Mix is available as a separate product (Cat No: PAN73028)
Activate: pre-heating step at 95° C for 10 minutes
Denature: 94° – 96° C
Extension: 72° C (allowing 15 - 30 seconds/Kb)

This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.
10x PAN Hot Start Buffer:160mM (NH4)2SO4, 1M Tris- HCl pH 8.3 and enhancers
PAN Hot Start DNA Polymerase can be stored for 12 months at -20° C.
20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1mM EDTA, 2 mM DTT, 50% Glycerol, and stabilizers.
Endonuclease and exonuclease activities were not detectable after 4 hours of incubation of 1 μg of pBR322 plasmid DNA and 0.5 μg Hind III-digested lambda phage DNA at 72° C in the presence of 20 u of PAN Hot Start.
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid-insoluble form in 30 minutes at 72° C.
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