PANScript red DNA Polymerase

 

PANScript red DNA Polymerase is a formulation of our regular PANScript DNA Polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides an easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing. When the reaction is complete, a sample of the reaction mix can be loaded directly onto the agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis. The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5 Units per 50 μl reaction.

The specificity and performance of PANScript red can be further improved with the use of 2x PAN Mate Additive (Cat No. PAN737041), which is designed for GC or ATrich DNA, dirty templates or sequences with a high level of secondary structure.
PANScript DNA Polymerase is purified from Thermus aquaticus.

 

DescriptionSizeProduct number
PANScript red DNA Polymerase 500 units ST-1100600
 
  • Easy visual recognition
  • Direct loading onto agarose gels
  • Same high performance as PANScript DNA Polymerase
  • Leaves “A” overhang
  • Available as a ready-to-use 2x reaction mix (PAN Mix Red)
  • Routine PCR assays
  • Products suitable for TA cloning
  • High throughput applications
10x NH4 Buffer 5 µl
50 mM MgCl2 Solution 1.5 - 4.0 µl
100 mM dNTP Mix (see below) 0.5 - 1.0 µl
Template and Primers as required
PANScript red 1.5 - 2.5 µl
Water (ddH2O) Up to 50 µl
100 mM dNTP Mix is available as a separate product (Cat No: PAN73028)

Denature: 94° – 96° C Elongate: 70° – 72° C (allowing 15 - 30 seconds/Kb) This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.
10x NH4 Reaction Buffer: 160 mM (NH4)2SO4, 670 mM
Tris-HCl (pH 8.8 at 25° C), 0.1% stabilizer
MgCl2 Stock Solution: 50 mM MgCl2 (suggested final concentration 1.5 mM - 4 mM).
20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA,
2 mM DTT, 50% Glycerol and stabilizers and inert dye.
PANScript red can be stored for 12 months at -20° C.
Endonuclease and exonuclease activities were not detectable after 2 and 1 hour incubations, respectively, of 1 μg lambda DNA and 0.22 μg of EcoR I-digested lambda DNA at 72° in the presence of 15 - 20 units of PANScript red DNA polymerase.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72° C.
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