PowerScript DNA Polymerase long range
PowerScript DNA Polymerase long range is a highperformance proprietary complex of enzymes specifically designed for difficult/problematic PCR applications requiring high processivity with fidelity that would normally fail with standard Taq polymerases. PowerScript DNA Polymerase long range is recommended for long Genomic DNA fragments of between 2 - 20 Kb, or up to 30 Kb Lambda DNA fragments. With Lambda DNA as template, the best performance is achieved in the 2 - 20 Kb range. PowerScript long is our original widely used PowerScript formulation.
|PowerScript DNA-Polymerase long range||250 units
- Ideal for problematic templates that fail with standard Taq DNA Polymerases
- Ideal for fragments 2 - 20 Kb in length
- Higher fidelity with Taq Polymerases
- Available as a ready-to-use 2x reaction mix
- For high fidelity PCR
- Suitable for both TA and blunt-end cloning
5x Hi-Spec Additive is a specificity enhancer. If necessary, re-dissolve Hi-Spec by heating to 70° C and vortexing.
20 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 2 mM DTT, 50% Glycerol, and stabilizers.
PowerScript long DNA Polymerase can be stored for 12 months at -20° C.
Endonuclease and exonuclease activities were not detectable after 4 hours of incubation of 1 μg of pBR322 plasmid DNA and 0.5 μg Hind III-digested Lambda DNA at 72° C in the Presence of 20 units of PowerScript long.
One unit is defined as the amount that incorporates 10nmoles of dNTPs into acid-precipitable form in 30 minutes at 72° C.