PowerScript DNA Polymerase short range

 

Powerscript short DNA Polymerase is a high-performance proprietary complex of enzymes specifically designed for difficult/problematic PCR applications requiring high processivity with fidelity that would normally fail with standard Taq Polymerases. PowerScript short DNA Polymerase is recommended for short genomic DNA fragments of up to 3 Kb, or up to 5 Kb on Lambda DNA.

Components                        250 Units       500 Units
PowerScript short range          62,5 μl          125 μl
10x OptiPuffer                         1,2 ml           2 x 1,2 ml
50 mM MgCI2 Solution             1,2 ml          1,2 ml
5x Hi-Spec Additive                  1,2 ml          1,2 ml
  
DescriptionSizeProduct number
PowerScript DNA-Polymerase short range 250 units
500 units
ST721064
ST721065
 
  • Ideal for problematic templates that fail with standard Taq DNA Polymerases
  • Amplifies genomic fragments up to 3 kb
  • Higher fidelity than Taq
  • For high fidelity PCR
  • Suitable for both TA and blunt-end cloning
5x Hi-Spec Additive is a specificity enhancer. If necessary, re-dissolve Hi-Spec by heating to 70° C and vortexing.
20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1mM EDTA, 2 mM DTT, 50% Glycerol, and stabilizers.
PowerScript short DNA Polymerase can be stored for 12 months at -20° C.
Endonuclease and exonuclease activities were not detectable after 4 hours of incubation of 1 μg of pBR322 plasmid DNA and 0.5 μg Hind III-digested Lambda DNA at 72° C in the presence of 20 units of PowerScript short.
One unit is defined as the amount that incorporates 10nmoles of dNTPs into acid-precipitable form in 30 minutes at 72° C.
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