Taq DNA Polymerases

 

Polymerases are enzymes that occur in all living organisms and catalyze the polymerization of nucleotides. Their function is the replication, i.e. the multiplication of the DNA in the S-phase, which is a precondition for the transmission of genetic material in the cell division.

A Taq DNA Polymerase is a thermostable DNA polymerase which transcribes exactly the template DNA (DNA orginale). The powder is optimized for a high selectivity during the amplification. The high processivity of Taq polymerase allows the amplification of DNA fragments up to 10kBasen.

We recommend the Taq Polymerase for the range of 100 bp to 8.000bp.
In addition, the Taq polymerase is suitable for DNA labeling with dUTP.

 

DescriptionSizeProduct number
Taq DNA Polymerase with buffer and MgCI2 250 units ST-30010250
Taq DNA Polymerase with buffer and MgCI2
with Phenol red
250 units

ST-30020250

 
20 mM Tris-HCI (pH 8.0), 100 mM KCL, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Nonidet P40 and 0.5% Tween 20.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble fraction in 30 minutes at 72° C under the standard assay conditions: 25 mM TAPS (tris-(hydrooxymethyl)- methyl-amino-propansulfonic acid, sodium salt) pH 9,3 (at 25° C), 50 mM KCl, 2 mM 50 mM MgCl2, 1 mM beta-mercaptoethanol, 200 μM each dATP, dGTP, dTTP, 100 μM dCTP (a mix of cold and P32-labelled), 12,5 μg activated salmon sperm DNA, in a final volume of 50 μl.
  • 10x PCR buffer with MgCl2: 100 mM Tris-HCI (pH 9.0 at 25° C), 500 mM KCl 15 mM MgCl2, 1.0% Triton X-100
  • 10x PCR buffer without MgCl2: 100 mM Tris-HCI (pH 9.0 at 25° C), 500 mM KCl 1.0% Triton X-100
  • Magnesium stock solution: 25 mM MgCl2
The enzyme is stable for more than 12 months if stored at -20° C.
The enzyme is also stable for some days at temperatures above 20° C.
Also a storage of up to three months at 8 ° C has no adverse effect on the activity of the polymerase under standard conditions.
Endonuclease and exonuclease activities were not detectable under the standard assay conditions

1. Incubation of 1μg PUC19 Vektor-DNA with 10 units Taq-Polymerase for 16 hours at 37°C.
2. Incubation of 1μg deoxyoligonukleotid (20 mer) with 50 units of enzymes for 6 hours at 37°C.
The Taq DNA Polymerase is a thermostable DNA polymerase from T. aquaticus of high purity with good fidelity and high processivity.
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