Laminin Mouse


This highly purified preparation of mouse Laminin I increases cell adhesion, migration, growth, and differentiation. It is composed of 111 chains with a total MW of 800 kD and is used for the coating of culture dishes.

Source: Murine Engelbreth-Holm-Swarm (EHS) tumor
Storage Buffer: Dulbecco‘s Modified Eagle‘s Medium with 10 μg/ml gentamycin sulfate
Storage: Store at -20° C or at -80° C in a manual defrost freezer
Purity: Purity > 90 % by SDS-PAGE


DescriptionSizeProduct number
Laminin from mouse 1 mg ST06-20501


Functional assays
Supports the formation of neuronal filaments of NG108-15 cells in a neurite outgrowth assay

Sterility testing
  • No bacterial or fungal growth detected after incubation at 37° C for 14 days following USP XXIV, Chapter 71 sterility testing
  • No mycoplasma contamination detected by PCR
  • Endotoxin concentrations < 20 EU/ml by LAL assay
The recommended working concentration is 0.05 - 10 µg/cm² of growth surface (0.05 - 10 µg/ml) depending on cell type.

a. Thaw stock solution on ice for several hours. Place plates on ice and pre-chill pipette tips.
Distribute the solution to completely cover the bottom of the wells.

b. The following table gives suggested volume required per well:
Plate TypeVolume Laminin per Well
6 wells (or 35 mm dish) 1 ml
24 wells 200 µl
48 wells 50 µl
48 wells 20 µl


c) Incubate the plates at 37° C for 1 hour. In the laminar flow hood, remove excess liquid from the wells of the tissue culture plate.

Rinse the wells once with tissue culture medium and then add your cells.
This website use cookies to create the most secure and effective website. By continuing to use this website you agree that cookies are used.
More information Ok