Accutase®

 

Accutase® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase® is a non-mammalian, non-bacterial ready-to-use solution.

PAN-Seratech produces and distributes Accutase® under license of the patentee Innovative Cell Technologies. Exclusive and for the whole of Europe.

 

DescriptionSizeProduct number
Accutase 100 ml
500 ml
ST10-21100
ST10-21500
 
Accutase® enzymes (activity > 500 U/ml) in DPBS w/o Ca/Mg with 0.5 mM EDTA and phenol red.
Accutase® can be used as a direct replacement of Trypsin for cell dissociation. Accutase® is a ready-to-use cell detachment solution made of collagenolytic and proteolytic enzymes. It is used for routine detachment of adherent cells from tissue culture plates and flasks. A multitude of cell types has been successfully sub-cultured with Accutase®, including fibroblasts, endothelial, vascular smooth muscle cells, as well as hepatocytes, embryonal stem cells, and many immortalized cell lines such as adherent CHO and BHK cells, HEK 293, L929, HeLa, 3T3, and others. Accutase® is free of mammalian or bacterial products.

Gentle detachment of cells for:
  • analysis of cell surface markers,
  • transfection procedures,
  • migration or proliferation assays,
  • flow cytometry,
  • and routine cell passage.
  • Ready-to-use solution
  • Gentle and effective cell detachment
  • Lower contamination risk
  • No neutralization required
  • Protection of surface epitopes
  • Maximum cell viability and enhanced plating efficiency
  • Alternative to Trypsin
For Accutase® references please see the Innovative Cell Technologies homepage: http://www.accutase.com/scientific-papers.html
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