Treated Sera

 

PAN-Seratech offers special types of serum: charcoal absorbed, delipidized, dialyzed, gamma irradiated, heat inactivated and gamma globulin reduced.

 

DescriptionSizeProduct number
Fetal Bovine Serum*, activated charcoal absorbed 100 ml
500 ml
ST30-2301
ST30-2302
Fetal Bovine Serum*, delipidized 100 ml
500 ml
ST30-3401
ST30-3402
Fetal Bovine Serum*, dialyzed 100 ml
500 ml
ST30-2101
ST30-2102
Fetal Bovine Serum*, gamma irradiated 100 ml
500 ml
ST30-2008
ST30-2009
FBS EU Professional*, gamma irradiated 100 ml
500 ml
ST30-2081
ST30-2085
Fetal Bovine Serum*, heat inactivated, Australia origin 100 ml
500 ml
ST30-1905
ST30-1906
Fetal Bovine Serum*, heat inactivated, EU origin 100 ml
500 ml
ST30-1908
ST30-1909
FBS EU Professional*, heat inactivated 100 ml
500 ml
ST30-1981
ST30-1985
Ultra low IgG*, Fetal bovine serum 100 ml
500 ml
ST30-2801
ST30-2802
Tetracycline-free serum*, Fetal bovine serum 100 ml
500 ml
ST30-3601
ST30-3602
 
* every origin is possible
 
 
Fetal bovine serum is heated in a water bath with dextran and activated charcoal. The activated charcoal, together with the substances bound to it, is then removed by centrifugation and filtration.

Application
• Work involving reduced hormone content (steroids)
• Work involving reduced growth factors (prevention of cell differentiation)
• Receptor studies (e. g. estrogens)
• Minimizes lot-to-lot variations in serum
Serum is dialyzed with a 10,000 Dalton exclusion membrane against physiological saline solution (alternative DPBS) until the glucose content is below 10 mg/100 ml.

Application
• Radioactive labeling studies
• Hormone-free applications
• Tests intolerant for small molecules such as nucleotides (hypoxanthine, thymidine), amino acids (serine, alanine etc.), sugars or metabolites Delipidized
  serum
Lipids are removed from serum by affinity chromatography.

Application
• Lipid metabolism studies
• Arteriosclerosis research

Serum is exposed to irradiation 25 kGy

Application
• Biopharmaceutical production
• Virus production
• Vaccine production
• Manufacturing of diagnostic products

Serum is heated for 30 min to 56 °C in a water bath under repeated gentle mixing.

Application
• Measurements of lactate dehydrogenase in the culture supernatant as a marker for cell damage (serum LDH is inactivated by heat)
• Minimizes lot-to-lot variations in serum (all thermolabile components are removed)
• Studies on vitamins and growth factors
• Enhance viral safety, since heat-labile viruses are inactivated
• Tests that do not tolerate presence of complement (complement destruction)
The average IgG level in fetal bovine serum is in the range ;of 70 to 330 μg/ml. The IgG content in our Ultra low IgG serum is reduced by affinity chromatography (protein-G affinity column) to max. 5 μg/ml. The biological activity of serum is not affected.

Application
• Antibody production
• Monoclonal antibodies
• Radioactive labeling

Fetal bovine serum is tested for absence of tetracycline using the TET-off system (luciferase).

Application
• TET-on / TET-off regulated gene expression
• Transfections
• Expression studies

 

 

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