Molecular Biology
Product details
Molecular Biology
Our products are produced with the highest standards made in Germany. We guarantee constant quality and full traceability. Furthermore, we also offer professional solutions for everthing to do with cell culture.
Introduction
Introduction
Background of Molecular Biology
The field of molecular biology overlaps with biology and chemistry and in particular, genetics and biochemistry. Molecular biology looks at the molecular mechanisms behind processes such as replication, transcription, translation and cell function. One way to describe the basis of molecular biology is to say it concerns understanding how genes are transcribed into RNA and how RNA is then translated into protein. PAN-Seratech offers a wide selection of products for molecular biology applications such as products suitable for TA cloning, products that are ideal for problematic templates that fail with standard TaqDNA Polymerases, RNA analyisis, DNA sequencing and others. The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA (in vitro) across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps involved in the PCR technique: DNA-denaturation, primer annealing, and extension.
The PCR is now a common and often indispensable technique, which is used in biological, medical and forensics laboratories for a huge number of different duties, for example, for the identification of hereditary diseases, infections and DNA analytics (genetic fingerprint, parental testing, phylogenetic tree). Nowadays the PCR counts to the most important methods of the modern molecular biology. PAN-Seratech offers different, specialized DNA Polymerases, Desoxy-Nucleotides, Polymerase-buffer solution, DNA Clean and various DNA-Markers.
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Polymerases
Polymerases
Taq DNA Polymerases
Polymerases are enzymes that occur in all living organisms and catalyze the polymerization of nucleotides. Their function is the replication, i.e. the multiplication of the DNA in the S-phase, which is a precondition for the transmission of genetic material in the cell division. | PANScript DNA Polymerase
PANScript is widely used by molecular biologists that have come to depend upon the robust performance of this reagent. PANScript is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. PANScript is a robust preparation and consistently delivers high yields with minimal background. PANScript possesses 5‘ to 3‘ exonuclease activity and leaves an “A” overhang such that the PCR product is suitable for effective integration into TA cloning vectors. | PANScript red DNA Polymerase
PANScript red DNA Polymerase is a formulation of our regular PANScript DNA Polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides an easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing. When the reaction is complete, a sample of the reaction mix can be loaded directly onto the agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis. The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5 Units per 50 μl reaction.
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PAN Hot Start DNA Polymerase
PAN Hot Start is a heat-activated thermostable DNA polymerase isolated from a novel organism. PAN Hot Start provides improved specificity as compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. | PowerScript DNA Polymerase short range
Powerscript short DNA Polymerase is a high-performance proprietary complex of enzymes specifically designed for difficult/problematic PCR applications requiring high processivity with fidelity that would normally fail with standard Taq Polymerases. PowerScript short DNA Polymerase is recommended for short genomic DNA fragments of up to 3 Kb, or up to 5 Kb on Lambda DNA.
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Molecular Biology Reagents
Molecular Biology Reagents
PAN-Seratech offers a broad range of high quality molecular biology reagents. You can find more information on the appropriate sites.
Proteinase K
Proteinase K is an enzyme used to digest most proteins in molecular-biological techniques. The enzyme may be used at 56° C for up to 4 hours, or 37° C for overnight incubations. Proteinase K solution is stabilized with a specially formulated buffer, and can be used directly from the freezer. | PAN Ladder I
PAN Ladder™ I is a popular ready-to-use molecular weight marker, especially designed for easy DNA quantification and size determination. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder I from the vial to the gel. PAN Ladder™ I produces a pattern of 14 regularly spaced bands, ranging from 200 to 10,000 bp. To allow easy identification and orientation, the 1000 and 10,000 bp bands have the highest intensity. When the standard loading volume of 5 μl per lane (720 ng of DNA) is being used, each band corresponds to a precise amount of DNA. A 5x sample loading buffer is supplied for your convenience. Under no circumstances should it be used to dilute/ load ladder.
| PAN DNA Clean
PAN DNA Clean is a novel, inexpensive solution, which provides a column-free method for nucleic-acid purification. Using a simple and rapid procedure, PAN DNA Clean can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates. |
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Agarose (molecular grade)
Agarose (DNase/RNase-free) is an extremely pure, high molecular biology grade agarose powder that has been extensively tested for RNase contamination. Agarose provides high resolution of DNA and RNA separated by electrophoresis and offers consistent resolution from lot to lot. | dNTP Sets
A set of ready-to-use molecular grade dNTP solutions consisting of 4 separate 100mM solutions of dATP, dGTP, dCTP and dTTP. For use in DNA polymerization reactions, DNA labelling and sequencing processes. Dependable PCR grade. All dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelf life due to the bacterio-static activity of Lithium towards various microorganisms. | dNTP Mix
A ready-to-use molecular grade dNTP Mix containing dATP, dCTP, dGTP and dTTP at pH 7.5 as Lithium salts in purified water. The mix is designed to save handson time for researchers and minimize the possibility of contamination. For use in DNA polymerization reactions, DNA labeling and sequencing processes. Dependable PCR grade. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelflife due to the bacteriostatic activity of Lithium towards various microorganisms. |
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